Human CD8+CTL Specific for the Mycobacterial Major Secreted Antigen 85A
نویسندگان
چکیده
منابع مشابه
Human CD8+ CTL specific for the mycobacterial major secreted antigen 85A.
The role of CD8(+) CTL in protection against tuberculosis in human disease is unclear. In this study, we stimulated the peripheral blood mononuclear cells of bacillus Calmette-Guérin (BCG)-vaccinated individuals with live Mycobacterium bovis BCG bacilli to establish short-term cell lines and then purified the CD8(+) T cells. A highly sensitive enzyme-linked immunospot (ELISPOT) assay for single...
متن کاملT-cell-epitope mapping of the major secreted mycobacterial antigen Ag85A in tuberculosis and leprosy.
Lymphoproliferation and gamma interferon (IFN-gamma) secretion in response to 28 overlapping 20-mer synthetic peptides covering the complete sequence of the mature (295-amino-acid) 85A component of the major secreted, fibronectin-binding antigen 85 complex from Mycobacterium tuberculosis and Mycobacterium bovis BCG (MTAg85A) was examined by using peripheral blood mononuclear cell (PBMC) culture...
متن کاملSynthetic peptides identify promiscuous human Th1 cell epitopes of the secreted mycobacterial antigen MPB70.
MPB70 is a secreted protein of Mycobacterium bovis and Mycobacterium tuberculosis which stimulates both cellular and humoral immune responses during infection with bovine and human tubercle bacilli. In addition, vaccination with MPB70 has been shown to induce Th1 cell responses and protection in animal models of tuberculosis. The present study was carried out to map the dominant human Th1 cell ...
متن کاملcharacterization of a monoclonal antibody specific for the parasite surface antigen-2 of leishmania major
the leishmania major parasite surface antigen-2 (psa-2) is a family of glycoinositol phospholipids anchored glycoprotoins expressed in both promastigotes and amastigotes. promastigote psa-2 comprises three polypeptides with approximate molecular weight of 96, 80 and 50 kda. amastigote express a distinct but closely psa-2 polypeptide with molecular weight of 50 kda. in this study fusion of sp2/0...
متن کاملImmune responses induced in cattle by vaccination with a recombinant adenovirus expressing Mycobacterial antigen 85A and Mycobacterium bovis BCG.
Cattle were vaccinated with an adenovirus expressing the mycobacterial antigen 85A (rAd85A), with Mycobacterium bovis BCG followed by rAd85A heterologous boosting, or with rAd85A followed by BCG boosting. BCG/rAd85A resulted in the highest direct gamma interferon responses. Cultured enzyme-linked immunospot assay analysis demonstrated that memory responses were induced by all three protocols bu...
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ژورنال
عنوان ژورنال: The Journal of Immunology
سال: 2000
ISSN: 0022-1767,1550-6606
DOI: 10.4049/jimmunol.165.12.7088